GEOTranscriptomicsPseudomonas putida KT2440Expression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE333646GEOGSEfalseTranscriptomic analysis of (S)-1,3-butanediol degradation pathway in Pseudomonas putida KT2440(S)-1,3-butanediol [(S)-1,3-BDO] is a chiral diol and stereoisomer of (R)-1,3-BDO with potential applications in the chemical and pharmaceutical industries; however, its microbial degradation pathway remains poorly understood. In this study, we performed strand-specific, paired-end RNA-seq transcriptomic analysis of Pseudomonas putida KT2440 grown on (S)-1,3-BDO and (S)-3-hydroxybutyrate [(S)-3-HB] as sole carbon sources to elucidate the underlying degradation pathway. Differential gene expression analysis was performed to identify genes specifically induced during (S)-1,3-BDO and (S)-3-HB metabolism, providing insight into the enzymatic steps and regulatory mechanisms governing their catabolism in P. putida KT2440.2026/06/01GSE333646GSM9770350GSM9770349GSM9770347GSM977034837044333646Pseudomonas putida KT2440