{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE333nnn/GSE333657/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE333657"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Effect of PSNEAT1 knockout on gene expression and ribosome biogenesis in NCI-H1299 cells","description":"Ribosomal DNA transcription is the initial and rate-limiting step of ribosome biogenesis and is tightly associated with nucleolar organization and tumor cell growth. PSNEAT1 is a nucleolar microprotein encoded by the NEAT1_1 transcript and has been shown to regulate rDNA transcription and nucleolar function. To investigate the transcriptional consequences of PSNEAT1 depletion, we generated PSNEAT1 knockout NCI-H1299 cells and performed RNA-seq analysis compared with control cells. Loss of PSNEAT1 resulted in broad gene expression changes associated with ribosome biogenesis, rRNA processing, nucleolar function, and cell growth-related pathways. These data provide a transcriptomic framework for understanding how PSNEAT1 contributes to nucleolar homeostasis and supports rDNA transcription-dependent tumor cell proliferation.","dates":{"publication":"2026/09/07"},"accession":"GSE333657","cross_references":{"GSM":["GSM9770547","GSM9770548","GSM9770549","GSM9770550","GSM9770551","GSM9770552"],"GPL":["24676"],"GSE":["333657"],"taxon":["Homo sapiens"]}}