GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE333nnn/GSE333657/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE333657GEOGSEfalseEffect of PSNEAT1 knockout on gene expression and ribosome biogenesis in NCI-H1299 cellsRibosomal DNA transcription is the initial and rate-limiting step of ribosome biogenesis and is tightly associated with nucleolar organization and tumor cell growth. PSNEAT1 is a nucleolar microprotein encoded by the NEAT1_1 transcript and has been shown to regulate rDNA transcription and nucleolar function. To investigate the transcriptional consequences of PSNEAT1 depletion, we generated PSNEAT1 knockout NCI-H1299 cells and performed RNA-seq analysis compared with control cells. Loss of PSNEAT1 resulted in broad gene expression changes associated with ribosome biogenesis, rRNA processing, nucleolar function, and cell growth-related pathways. These data provide a transcriptomic framework for understanding how PSNEAT1 contributes to nucleolar homeostasis and supports rDNA transcription-dependent tumor cell proliferation.2026/09/07GSE333657GSM9770547GSM9770548GSM9770549GSM9770550GSM9770551GSM977055224676333657Homo sapiens