{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE333nnn/GSE333803/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE333803"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Distinct media requirements for dopaminergic neuron reprogramming from human glia and fibroblasts","description":"Bulk RNA sequencing was performed on human glial progenitor cells (hGPCs) derived from the hESC line RC17 and reprogrammed toward a dopaminergic neuron fate using Ascl1, Lmx1a, Nurr1 and shREST (ALNRi). Cells were cultured under two media conditions; standard NDiff medium or a B27/Neurobasal-based medium without small molecules (new media) and harvested at day 10 and day 21 post-transduction (three technical replicates per condition). The dataset was generated to identify transcriptional differences underlying the improved dopaminergic reprogramming efficiency observed with the new media. Gene-level counts were quantified using Salmon (alignment-based mode, GRCh38/hg38) and merged across samples using tximport.","dates":{"publication":"2026/08/21"},"accession":"GSE333803","cross_references":{"GSM":["GSM9773572","GSM9773571","GSM9773574","GSM9773573","GSM9773570","GSM9773581","GSM9773580","GSM9773579","GSM9773576","GSM9773575","GSM9773578","GSM9773577"],"GPL":["24676"],"GSE":["333803"],"taxon":["Homo sapiens"],"PMID":["[42608437]"]}}