GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE333nnn/GSE333803/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE333803GEOGSEfalseDistinct media requirements for dopaminergic neuron reprogramming from human glia and fibroblastsBulk RNA sequencing was performed on human glial progenitor cells (hGPCs) derived from the hESC line RC17 and reprogrammed toward a dopaminergic neuron fate using Ascl1, Lmx1a, Nurr1 and shREST (ALNRi). Cells were cultured under two media conditions; standard NDiff medium or a B27/Neurobasal-based medium without small molecules (new media) and harvested at day 10 and day 21 post-transduction (three technical replicates per condition). The dataset was generated to identify transcriptional differences underlying the improved dopaminergic reprogramming efficiency observed with the new media. Gene-level counts were quantified using Salmon (alignment-based mode, GRCh38/hg38) and merged across samples using tximport.2026/08/21GSE333803GSM9773572GSM9773571GSM9773574GSM9773573GSM9773570GSM9773581GSM9773580GSM9773579GSM9773576GSM9773575GSM9773578GSM977357724676333803Homo sapiens[42608437]