{"database":"GEO","file_versions":[],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Homo sapiens"],"gds_type":["Non-coding RNA profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE333874"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Small RNA sequencing reveals placental miRNAs linked to congenital transmission of Trypanosoma cruzi","description":"Background: Congenital transmission remains an important source of new Chagas disease cases. This study aimed to identify placental microRNAs (miRNAs) associated with congenital transmission of Trypanosoma cruzi. Methods: Placental samples from T. cruzi-infected mothers were classified according to congenital transmission status and analyzed by small RNA sequencing. Differential expression was assessed using DESeq2 with Benjamini-Hochberg false discovery rate correction. Nine candidate miRNAs were subsequently assessed by RT-qPCR. Results: Small RNA sequencing was performed in 31 placental samples, including 13 from transmitting mothers and 18 from non-transmitting mothers. hsa-miR-155-5p was the only miRNA significantly upregulated in placentas from transmitting mothers after multiple-testing correction and was selected as a candidate for further validation. Seven additional miRNAs (hsa-miR-193a-5p, hsa-miR-187-3p, hsa-miR-512-5p, hsa-miR-515-5p, hsa-miR-526a-5p, hsa-miR-99b-3p, and hsa-miR-455-5p) were selected based on nominal p-value, expression abundance, and differences in mean normalized counts between groups. RT-qPCR assessment in 15 placental samples, including 5 from transmitting mothers and 10 from non-transmitting mothers, showed higher normalized expression of hsa-miR-193a-5p and hsa-miR-187-3p in transmitting mothers.Conclusion: Placental miRNA profiling identified hsa-miR-155-5p, hsa-miR-193a-5p, and hsa-miR-187-3p as candidates for further investigation in congenital transmission of T. cruzi. These findings require confirmation in larger cohorts.","dates":{"publication":"2026/06/04"},"accession":"GSE333874","cross_references":{"GSM":["GSM9776360","GSM9776367","GSM9776345","GSM9776368","GSM9776346","GSM9776365","GSM9776366","GSM9776363","GSM9776364","GSM9776361","GSM9776362","GSM9776349","GSM9776347","GSM9776369","GSM9776348","GSM9776370","GSM9776371","GSM9776356","GSM9776357","GSM9776354","GSM9776355","GSM9776374","GSM9776352","GSM9776353","GSM9776375","GSM9776350","GSM9776372","GSM9776373","GSM9776351","GSM9776358","GSM9776359"],"GPL":["24676"],"GSE":["333874"],"taxon":["Homo sapiens"]}}