GEOGenomicsHomo sapiensNon-coding RNA profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE333874GEOGSEfalseSmall RNA sequencing reveals placental miRNAs linked to congenital transmission of Trypanosoma cruziBackground: Congenital transmission remains an important source of new Chagas disease cases. This study aimed to identify placental microRNAs (miRNAs) associated with congenital transmission of Trypanosoma cruzi. Methods: Placental samples from T. cruzi-infected mothers were classified according to congenital transmission status and analyzed by small RNA sequencing. Differential expression was assessed using DESeq2 with Benjamini-Hochberg false discovery rate correction. Nine candidate miRNAs were subsequently assessed by RT-qPCR. Results: Small RNA sequencing was performed in 31 placental samples, including 13 from transmitting mothers and 18 from non-transmitting mothers. hsa-miR-155-5p was the only miRNA significantly upregulated in placentas from transmitting mothers after multiple-testing correction and was selected as a candidate for further validation. Seven additional miRNAs (hsa-miR-193a-5p, hsa-miR-187-3p, hsa-miR-512-5p, hsa-miR-515-5p, hsa-miR-526a-5p, hsa-miR-99b-3p, and hsa-miR-455-5p) were selected based on nominal p-value, expression abundance, and differences in mean normalized counts between groups. RT-qPCR assessment in 15 placental samples, including 5 from transmitting mothers and 10 from non-transmitting mothers, showed higher normalized expression of hsa-miR-193a-5p and hsa-miR-187-3p in transmitting mothers.Conclusion: Placental miRNA profiling identified hsa-miR-155-5p, hsa-miR-193a-5p, and hsa-miR-187-3p as candidates for further investigation in congenital transmission of T. cruzi. These findings require confirmation in larger cohorts.2026/06/04GSE333874GSM9776360GSM9776367GSM9776345GSM9776368GSM9776346GSM9776365GSM9776366GSM9776363GSM9776364GSM9776361GSM9776362GSM9776349GSM9776347GSM9776369GSM9776348GSM9776370GSM9776371GSM9776356GSM9776357GSM9776354GSM9776355GSM9776374GSM9776352GSM9776353GSM9776375GSM9776350GSM9776372GSM9776373GSM9776351GSM9776358GSM977635924676333874Homo sapiens