GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE334nnn/GSE334010/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE334010GEOGSEfalseTranscriptional and spatial profiling of fibroblasts from human lungs highlights CTHRC1+ cells as fibrogenic signaling hubs in fibrosis [scRNA-seq culture]This dataset contains single-cell RNA sequencing (scRNA-seq) data from primary human lung fibroblasts isolated from idiopathic pulmonary fibrosis (IPF) and age-matched control lungs expanded under standard in vitro culture conditions using three isolation protocols: outgrowth, whole lung cell suspension (WLCS), and negative fraction. Droplet-based scRNA-seq (10x Genomics Chromium) profiling across multiple passages identified seven transcriptionally distinct fibroblast subtypes. Differential gene expression and pathway enrichment analyses revealed broad culture-induced ECM-producing and proliferative transcriptional adaptations alongside partial retention of subtype-specific programs. This dataset provides a resource for investigating the transcriptional consequences of in vitro culture on human lung fibroblast heterogeneity and disease-associated mesenchymal states in IPF.2026/08/19GSE334010GSM9778887GSM9778876GSM9778875GSM9778886GSM9778885GSM9778874GSM9778884GSM9778873GSM9778883GSM9778882GSM9778881GSM9778880GSM9778879GSM9778878GSM9778888GSM977887724676334010Homo sapiens