{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE334nnn/GSE334037/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Danio rerio"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE334037"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell RNA sequencing reveals the transcriptional landscape of intestinal lymphoid cells in zebrafish challenged with SVCV","description":"How diffuse gut-associated lymphoid tissues (GALT) in bony fish coordinate efficient local antiviral defense without encapsulated lymph nodes and germinal centers remains an evolutionary mystery. Here, utilizing a zebrafish model of intestinal spring viremia of carp virus (SVCV) infection, we profiled 46,606 single mucosal immune cells at 14 days post-infection. By comprehensively defining the T and B cell compartments, we demonstrated a high evolutionary similarity in immune cell composition between teleosts and mammals. Single-cell transcriptomics further revealed a specialized antiviral functional division paradigm: cd8+ T cells mediate frontline cytotoxic killing via isg15+ clusters, while cd4+ Th17 cells (rorc+/il17a/f3+) repair the epithelial barrier. Moreover, intercellular communication and functional assays showed that macrophages and T cells deliver MIF signals via CD74/CXCR4 to compensate for anatomical defects, driving the local survival and clonal expansion of IgM+ B cells outside germinal centers. Together, these findings reveal how decentralized mucosal immunity provides robust local protection in jawed vertebrates.","dates":{"publication":"2026/08/31"},"accession":"GSE334037","cross_references":{"GSM":["GSM9779537","GSM9779538","GSM9779539","GSM9779540","GSM9779541","GSM9779536"],"GPL":["30277"],"GSE":["334037"],"taxon":["Danio rerio"]}}