GEOGenomicsHomo sapiensGenome binding/occupancy profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE334084GEOGSEfalseArginine methylationof BAF155 regualtes interactions with RNA processing machinery [CUT&RUN]Post-translational modifications (PTMs) of chromatin remodelers are abundant but functionally understudied. Here we investigate the role of asymmetric dimethylation of arginine 1064 (BAF155me2a) on the SWI/SNF core subunit BAF155, a mark deposited by CARM1/PRMT4 that has been linked to tumor progression but whose molecular function remains unclear. Using immunoprecipitation–mass spectrometry with a dimethyl-specific antibody, we found that R1064me2 selectively enhances BAF155 interactions with RNA processing factors, including the anti-termination protein SCAF4, splicing factors, and the transcription factor RFX5. CUT&RUN profiling showed that BAF155me2a, SCAF4, and RFX5 co-occupy promoter regions, and reciprocal immunoprecipitations confirmed that the SCAF4–BAF155 interaction depends on R1064 methylation. To test the functional consequences of this modification, we generated cells expressing either wild-type BAF155 or a methylation-deficient BAF155-R1064K mutant. Loss of methylation did not alter chromatin accessibility, BAF155 genomic occupancy, or SCAF4 recruitment. However, nascent transcription measured by TT-seq revealed a coordinated reduction in 5′ sense transcripts and upstream antisense transcripts (PROMPTs) at BAF155-bound promoters, with a quantitatively larger decrease in PROMPTs at SCAF4 co-bound sites. The effect was restricted to the promoter-proximal region and resolved toward the gene end, consistent with a defect in productive elongation downstream of RNA polymerase II recruitment. These data support a model in which BAF155 dimethylation provides a co-transcriptional interface coupling SWI/SNF to RNA processing machinery, and identify regulation of nascent transcription as a non-canonical function of SWI/SNF PTMs.2026/06/03GSE334084GSM9780180GSM9780181GSM9780184GSM9780185GSM9780182GSM9780183GSM9780188GSM9780189GSM9780186GSM9780187GSM9780148GSM9780149GSM9780146GSM9780147GSM9780191GSM9780192GSM9780190GSM9780195GSM9780151GSM9780196GSM9780152GSM9780193GSM9780150GSM9780194GSM9780155GSM9780156GSM9780153GSM9780197GSM9780198GSM9780154GSM9780159GSM9780157GSM9780158GSM9780162GSM9780163GSM9780160GSM9780161GSM9780166GSM9780167GSM9780164GSM9780165GSM9780168GSM9780169GSM9780170GSM9780173GSM9780174GSM9780171GSM9780172GSM9780177GSM9780178GSM9780175GSM9780176GSM978017934284334084Homo sapiens