GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE334nnn/GSE334312/primaryOK200TranscriptomicsStreptococcus pyogenesExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE334312GEOGSEfalseSpxA1 and SpxA2 Function as a Stoichiometry-Dependent Regulatory Rheostat Governing Virulence Gene Expression in Group A Streptococcus — RNA-seq datasetGroup A Streptococcus (GAS) encodes two paralogs of the Spx transcriptional regulator, SpxA1 and SpxA2, which interact with RNA polymerase to modulate virulence gene expression. To define the transcriptomic consequences of SpxA1 and SpxA2 loss, we performed strand-non-specific paired-end RNA-seq in the wild-type emm3 GAS strain MGAS10870 and isogenic deletion mutants (deltaSpxA1 and deltaSpxA2) grown to mid-exponential phase in Todd-Hewitt broth supplemented with 0.2% yeast extract (THY). Differential expression analysis identified distinct transcriptomic signatures for each paralog, with SpxA1 primarily influencing oxidative stress response genes and SpxA2 modulating CovR-regulated virulence factor-encoding genes. These data contribute to a multi-omic analysis including DIA proteomics, NanoString nCounter, and CovR ChIP-exo that defines the regulatory roles of SpxA1 and SpxA2 in GAS pathogenesis.2026/07/13GSE334312GSM9785264GSM9785265GSM9785266GSM9785260GSM9785261GSM9785262GSM9785263GSM9785258GSM978525928678334312Streptococcus pyogenes