GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE334nnn/GSE334479/primary200OKMethylation profilingVigna radiataMethylation profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE334479GEOGSEfalseWhole-genome bisulfite sequencing reveals dynamic DNA methylation changes in Vigna radiata under direct and pre-conditioned salt stressDNA methylation is an important epigenetic mechanism regulating gene expression and stress memory in plants. To investigate the role of DNA methylation in salt stress adaptation, we performed whole-genome bisulfite sequencing (WGBS) of mung bean (Vigna radiata cv. Weilv 9) leaves under two treatment regimes: T1 (pre-conditioned with 100 mM NaCl for 24 h, recovered for 48 h, then re-exposed to 100 mM NaCl) and T2 (direct exposure to 100 mM NaCl without preconditioning). Seedlings were grown hydroponically in half-strength Murashige and Skoog medium under controlled environmental conditions. Genomic DNA was extracted from the first fully expanded true leaves at 0, 6, and 48 h after the onset of the final salt treatment, with three biological replicates per condition. Bisulfite-converted DNA was sequenced to generate genome-wide methylation profiles. Comparative analysis revealed dynamic changes in CG, CHG, and CHH contexts, providing insights into the epigenetic basis of salt tolerance and stress priming in legumes.2026/06/07GSE334479GSM9789424GSM9789425GSM9789422GSM9789423GSM9789431GSM9789420GSM9789432GSM9789421GSM9789430GSM9789419GSM9789428GSM9789417GSM9789418GSM9789429GSM9789415GSM9789426GSM9789416GSM978942737083334479Vigna radiata