GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE334nnn/GSE334597/primaryOK200OtherMus musculusOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE334597GEOGSEfalseTranslatomic analysis of the wild-type (WT) and total body eIF2A-KO mouse liver tissueTranslation initiation in eukaryotes is a complex process involving more than 12 initiation factors. The heterotrimeric factor eIF2 (α, β, γ) is the primary mediator of methionyl-initiator tRNA (Met-tRNAi) delivery to the 40S ribosomal subunit. eIF2A is an alternative factor capable of mediating this delivery and is thought to function in non-canonical translation initiation pathways. To investigate the physiological role of eIF2A in mammals, our laboratory generated a total eIF2A knockout (KO) mouse model. Although eIF2A-KO mice are viable, they exhibit features of metabolic syndrome. To examine translatomic alterations associated with eIF2A deficiency, we performed ribosome profiling on liver tissues from wild-type (WT) and eIF2A-KO mice. High-throughput sequencing was used to identify affected molecular pathways and to define the role of eIF2A in metabolic homeostasis.2026/09/16GSE334597GSM9791579GSM9791580GSM9791581GSM9791582GSM9791583GSM979158424247334597Mus musculus[42712787]