{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE334nnn/GSE334799/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":[" Other","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE334799"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"3' end sequencing of HeLa-NXF1-dTAG cells upon NXF1 degradation to characterize alternative polyadenylation dynamics","description":"Nuclear export factor 1 (NXF1) is the principal mRNA export receptor in eukaryotes. To characterize the role of NXF1 in regulating alternative polyadenylation (APA) dynamics, we employed the dTAG degradation system in a HeLa-NXF1-dTAG stable cell line. Cells were treated with 500 nM dTAG-13 or DMSO (vehicle control) for 6 hours to induce acute NXF1 depletion. Total RNA was subjected to 3' end sequencing (QuantSeq 3' mRNA-Seq Library Prep Kit V2, Lexogen) for APA analysis. Each condition was performed in biological duplicate.","dates":{"publication":"2026/06/14"},"accession":"GSE334799","cross_references":{"GSM":["GSM9797165","GSM9797164","GSM9797163","GSM9797162","GSM9802926","GSM9802925","GSM9802924","GSM9802923"],"GPL":["29480"],"GSE":["334799"],"taxon":["Homo sapiens"]}}