GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE334nnn/GSE334820/primary200OKGenomicsMus musculusGenome binding/occupancy profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE334820GEOGSEfalseAging is associated with enhanced chromatin compaction, global loss of H3 acetylation and local changes in transcription [CUT&Tag]Advanced age is associated with specific alterations in organismic and cellular identity and behavior. To gain insights into age-associated rod photoreceptor function decline, we generated multi-omic datasets including 3D-genome topology, histone marks, chromatin accessibility, DNA methylation and transcriptome of rods from young- and aged-mice. We show global chromatin compaction during aging with regional alterations enriched at active chromatin. High-resolution microscopy leveraging the inverted nuclear architecture of mouse rods validates these findings. Chromatin dynamics broadly associate with epigenomic and transcriptional changes. Notably, a megabase-sized region presents multi-level alterations, with de novo transcription of a locus containing an open reading frame (ORF). This ORF encodes a functional histone acetyltransferase-inhibitor domain which transcription parallels H3 acetylation loss. Finally, we identify multiple highly significant local alterations of transcription at non-annotated regions and age-related macular degeneration genes. Our studies link age-related chromatin landscape changes and gene expression, potentially influencing rod function and vulnerability to diseases.2026/09/09GSE334820GSM9797819GSM9797818GSM9797817GSM9797816GSM9797800GSM9797821GSM9797820GSM9797804GSM9797803GSM9797802GSM9797801GSM9797808GSM9797807GSM9797806GSM9797805GSM9797809GSM9797796GSM9797811GSM9797799GSM9797810GSM9797798GSM9797797GSM9797815GSM9797814GSM9797813GSM979781230172334820Mus musculus[42687910]