GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE334nnn/GSE334821/primaryOK200OtherMus musculusOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE334821GEOGSEfalseAging is associated with enhanced chromatin compaction, global loss of H3 acetylation and local changes in transcription [Hi-C]Advanced age is associated with specific alterations in organismic and cellular identity and behavior. To gain insights into age-associated rod photoreceptor function decline, we generated multi-omic datasets including 3D-genome topology, histone marks, chromatin accessibility, DNA methylation and transcriptome of rods from young- and aged-mice. We show global chromatin compaction during aging with regional alterations enriched at active chromatin. High-resolution microscopy leveraging the inverted nuclear architecture of mouse rods validates these findings. Chromatin dynamics broadly associate with epigenomic and transcriptional changes. Notably, a megabase-sized region presents multi-level alterations, with de novo transcription of a locus containing an open reading frame (ORF). This ORF encodes a functional histone acetyltransferase-inhibitor domain which transcription parallels H3 acetylation loss. Finally, we identify multiple highly significant local alterations of transcription at non-annotated regions and age-related macular degeneration genes. Our studies link age-related chromatin landscape changes and gene expression, potentially influencing rod function and vulnerability to diseases.2026/09/09GSE334821GSM9797829GSM9797828GSM9797827GSM9797822GSM9797831GSM9797830GSM9797826GSM9797825GSM9797824GSM97978231702130172334821Mus musculus[42687910]