GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE334nnn/GSE334921/primary200OKGenomicsMus musculusNon-coding RNA profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE334921GEOGSEfalseSmall non-coding RNA profiling in the hippocampus of mice with postoperative cognitive dysfunctionPostoperative cognitive dysfunction (POCD) commonly affects elderly surgical patients and is associated with impairments in memory, attention, and executive function that can substantially reduce quality of life. The precise pathogenesis of POCD remains unclear, although trauma-induced epigenetic mechanisms have been implicated. Conventional sequencing methods are hindered by reverse-transcription arrest at modified RNAs, which may result in the underrepresentation or loss of small noncoding RNAs (sncRNAs) with important regulatory functions. Here, we applied panoramic RNA display by overcoming RNA modification-induced aborted sequencing (PANDORA-seq) to generate a comprehensive profile of highly modified sncRNAs in the hippocampus of mice with surgery-induced cognitive impairment. The dataset profiles the composition of hippocampal sncRNA classes—including miRNAs, tsRNAs, rsRNAs, and piRNAs—in control and surgery-exposed mice during the early postoperative period. We performed sequencing quality-control analyses and, as an orthogonal, assay-level check on selected sequencing signals, quantified selected tsRNAs by qRT-PCR. Structural mapping of these tsRNAs to their parental tRNAs was also performed. Overall, this resource provides a foundation for investigating neuroepigenetic changes associated with early postoperative cognitive impairment and for generating hypotheses regarding the molecular mechanisms of POCD.2026/09/25GSE334921GSM9802149GSM9802148GSM9802147GSM9802146GSM9802145GSM9802144GSM9802143GSM980214237458334921Mus musculus