GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE334nnn/GSE334949/primaryOK200TranscriptomicsDrosophila melanogasterExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE334949GEOGSEfalseNotch Signaling Reprograms Glial Lipid Metabolism to Promote Hypoxia ResistanceTo understand the transcriptional basis of Notch activation-conferred hypoxia tolerance, we performed single-nucleus RNA sequencing (snRNA-seq) of the developing Drosophila central nervous system and generated a comprehensive cellular atlas at single-cell resolution. Analysis of Eaat1-expressing cells revealed that Notch activation reshaped hypoxia-induced transcriptional responses and counteracted metabolic suppression caused by low oxygen. Together, our findings identify Eaat1 glia as a metabolically adaptive glial population and reveal a conserved Notch-regulated mechanism that rewires lipid metabolism to support hypoxia tolerance in the developing brain.2026/06/26GSE334949GSM9802556GSM9802555GSM9802554GSM980255334457334949Drosophila melanogaster