{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE334nnn/GSE334998/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Genomics"],"species":["Drosophila melanogaster"],"gds_type":["Genome binding/occupancy profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE334998"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Transcription machinery clustering by Integrator synchronizes histone gene expression","description":"Numerous components of the transcription machinery, including RNA polymerase II (Pol II), accumulate in high-concentration clusters at gene promoters. Whether these clusters assemble on demand during transcription or constitute regulatory units remains unclear. Examining the Drosophila histone locus—where histone genes normally transcribe exclusively during S phase—we unexpectedly found large clusters containing non-chromatin-bound Pol II and elongation factors outside of S phase. When transcription activates during S phase, P-TEFb drives an accelerated turnover of the cluster components. Dispersion of clusters through depletion of the Integrator complex endonuclease module causes histone transcription to have a diminished S-phase peak and instead occur ectopically throughout the cell cycle. We propose that clusters constitute gate-keeping hubs that maintain a poised machinery pool, restricting gene activation to defined temporal windows.","dates":{"publication":"2026/06/10"},"accession":"GSE334998","cross_references":{"GSM":["GSM9803169","GSM9803168","GSM9803181","GSM9803180","GSM9803167","GSM9803166","GSM9803165","GSM9803164","GSM9803163","GSM9803162","GSM9803161","GSM9803160","GSM9803159","GSM9803158","GSM9803179","GSM9803157","GSM9803170","GSM9803178","GSM9803156","GSM9803155","GSM9803177","GSM9803176","GSM9803154","GSM9803175","GSM9803153","GSM9803152","GSM9803174","GSM9803173","GSM9803151","GSM9803172","GSM9803150","GSM9803171"],"GPL":["22106"],"GSE":["334998"],"taxon":["Drosophila melanogaster"]}}