{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE335nnn/GSE335071/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Other"],"species":["synthetic construct"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE335071"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Direct RNA sequencing of in vitro transcribed eGFP mRNA modified with 5-methoxyuracil","description":"The aim of this study was to understand potential biases in the incorporation of modified nucleotides, specifically 5-methoxyuracil, in in vitro transcription reactions by different T7 polymerases through Nanopore Direct RNA Sequencing. Incorporation by a range of T7 polymerases using different DNA templates was sequenced using RNA004 flow cell chemistry. Analysis was performed using basecall error-based modification detection (EpiNano).","dates":{"publication":"2026/08/20"},"accession":"GSE335071","cross_references":{"GSM":["GSM9805520","GSM9805521","GSM9805522","GSM9805523","GSM9805524","GSM9805525"],"GPL":["25738"],"GSE":["335071"],"taxon":["synthetic construct"]}}