GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE335nnn/GSE335071/primary200OKOthersynthetic constructOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE335071GEOGSEfalseDirect RNA sequencing of in vitro transcribed eGFP mRNA modified with 5-methoxyuracilThe aim of this study was to understand potential biases in the incorporation of modified nucleotides, specifically 5-methoxyuracil, in in vitro transcription reactions by different T7 polymerases through Nanopore Direct RNA Sequencing. Incorporation by a range of T7 polymerases using different DNA templates was sequenced using RNA004 flow cell chemistry. Analysis was performed using basecall error-based modification detection (EpiNano).2026/08/20GSE335071GSM9805520GSM9805521GSM9805522GSM9805523GSM9805524GSM980552525738335071synthetic construct