{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Tabular":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE335nnn/GSE335221/suppl/GSE335221_featurecounts.readcounts.ann.tsv.gz","ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE335nnn/GSE335221/suppl/GSE335221_featurecounts.readcounts.ann_1.tsv.gz"],"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE335nnn/GSE335221/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE335221"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Effect of keratinocyte-intrinsic VISTA deletion on UV-induced type I interferon and inflammatory gene expression in mouse epidermis [bulk RNA-seq]","description":"(abstract)Type I interferon (IFN-I) signaling is a central driver of cutaneous lupus erythematosus (CLE), but the keratinocyte-intrinsic checkpoints that restrain UV-triggered IFN-I responses remain poorly defined. Here we report that the immune checkpoint VISTA (encoded by Vsir) is expressed in epidermal keratinocytes and acts cell-intrinsically to suppress cGAS–STING–dependent IFN-I induction following ultraviolet (UV) exposure. Conditional deletion of Vsir in keratinocytes (K14Cre-Vsir^fl/fl^) results in markedly elevated expression of interferon-stimulated genes (ISGs), chemokines, and antigen presentation machinery at baseline and is further amplified by acute UVB irradiation. Co-deletion of STING (Sting1) abrogates this hyperinflammatory transcriptional program, placing VISTA upstream of the cGAS–STING–IFN-I axis. Mechanistically, our data support a model in which VISTA restrains EGFR/LRIG1/NUMB-dependent stabilization of phosphorylated (pY245) STING, thereby tuning the magnitude of UV-induced IFN-I output. These findings identify a keratinocyte-intrinsic role for VISTA in suppressing sterile cutaneous inflammation and have translational relevance for photosensitive autoimmune skin disease and for cutaneous immune-related adverse events of VISTA-targeted therapies.","dates":{"publication":"2026/06/16"},"accession":"GSE335221","cross_references":{"GSM":["GSM9808376","GSM9808377","GSM9808374","GSM9808375","GSM9808358","GSM9808359","GSM9808378","GSM9808379","GSM9808372","GSM9808373","GSM9808370","GSM9808371","GSM9808365","GSM9808366","GSM9808385","GSM9808363","GSM9808364","GSM9808369","GSM9808367","GSM9808368","GSM9808380","GSM9808383","GSM9808361","GSM9808362","GSM9808384","GSM9808381","GSM9808382","GSM9808360"],"GPL":["30172"],"GSE":["335221"],"taxon":["Mus musculus"]}}