GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE335nnn/GSE335248/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE335248GEOGSEfalseRNA-seq analysis of U251 cells following tRF-3021a knockdown at 8 and 48 hourstRNA-derived fragments (tRFs) are relatively recently discovered small RNAs implicated in gene-regulatory processes in diverse biological contexts, but there have been few reports of clear phenotypic roles for these small RNAs in cancer progression. High expression of the 3' tRFs tRF-3009a, tRF-3021a, and tRF-3030a is associated with poor overall survival in low-grade glioma. In glioblastoma cells, these tRFs enhance invasion and migration, while tRF-3021a is uniquely required for cell proliferation, suppression of apoptosis, and maintenance of global protein synthesis. This RNA-seq study profiles transcriptomic changes following tRF-3021a knockdown in U251 cells at early and late time points (8 hours and 48 hours)2026/09/25GSE335248GSM9809003GSM9809004GSM9809001GSM9809002GSM9808996GSM9808997GSM9808994GSM9809005GSM9808995GSM9808998GSM9808999GSM98090003428434281335248Homo sapiens