{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE335nnn/GSE335441/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE335441"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"HCFC2 knockdown in human colorectal cancer cell lines","description":"There is an urgent and unmet need for improved therapeutic approaches against aggressive colorectal cancer (CRC). Host Cell Factor 2 (HCF-2) is a transcriptional coactivator with an emerging role in cancer biology. Our data suggest that HCF-2 is a critical effector of peroxisome proliferator-activated receptor-gamma coactivator 1 beta (PGC-1β) signaling in CRC, supporting its potential as a targetable vulnerability in aggressive disease. Previous findings from our lab show that shRNA-mediated depletion of HCF-2 decreases anchorage-independent growth in CRC models, indicating a role for HCF-2 in CRC cell survival. To identify transcriptional programs regulated downstream of HCF-2, we performed RNA-seq following shRNA-mediated HCF-2 knockdown in SW620 and T84 CRC cell lines. Knockdown efficiency was confirmed by western blot prior to sample submission, and RNA-seq data were provided to collaborators for downstream computational analysis. The resulting transcriptomic profile will help define HCF-2-dependent gene expression programs and potential targetable vulnerabilities in aggressive CRC.","dates":{"publication":"2026/06/15"},"accession":"GSE335441","cross_references":{"GSM":["GSM9813832","GSM9813831","GSM9813842","GSM9813834","GSM9813833","GSM9813841","GSM9813830","GSM9813840","GSM9813829","GSM9813828","GSM9813839","GSM9813825","GSM9813836","GSM9813835","GSM9813838","GSM9813827","GSM9813826","GSM9813837"],"GPL":["34281"],"GSE":["335441"],"taxon":["Homo sapiens"]}}