{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE335nnn/GSE335452/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":[" Other","Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE335452"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell RNA-seq and spatial transcriptomics characterize CD8+ exhausted T cells in pancreatic ductal adenocarcinoma","description":"Pancreatic cancer resists immunotherapy due to a suppressive immune microenvironment where CD8⁺ T cells play a key role. Using single‑cell RNA sequencing and spatial transcriptomics, we characterized CD8⁺ exhausted T (Tex) cells in pancreatic ductal adenocarcinoma (PDAC). We generated single‑cell profiles from PDAC tumors and matched peripheral blood mononuclear cells, and performed T cell sub‑analysis. We found CXCL13 upregulated and GZMK downregulated in CD8⁺ Tex cells. Cell‑cell interaction analysis showed that T cells most frequently interacted with myeloid cells and cancer cells via ligand‑receptor pairs; INHBA⁺ macrophages and cancer cells communicated most with CD8⁺ Tex cells. Two key LR pairs (SPP1–integrin α4β1 and PLAUR–integrin α4β1) mediated crosstalk between cancer cells and CD8⁺ Tex cells, confirmed by immunofluorescence, spatial mapping, and protein docking. High SPP1 and PLAUR expression correlated with poor prognosis in TCGA‑PAAD. These findings provide a resource for understanding CD8⁺ T cell exhaustion in PDAC.","dates":{"publication":"2026/06/16"},"accession":"GSE335452","cross_references":{"GSM":["GSM9813931","GSM9813920","GSM9813930","GSM9813922","GSM9813933","GSM9813921","GSM9813932","GSM9813928","GSM9813927","GSM9813919","GSM9813929","GSM9813924","GSM9813923","GSM9813926","GSM9813925"],"GPL":["24676"],"GSE":["335452"],"taxon":["Homo sapiens"]}}