GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE335nnn/GSE335556/primaryOK200TranscriptomicsPseudomonas aeruginosaExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE335556GEOGSEfalseA Pseudomonas aeruginosa LysR-type transcriptional regulator, PA3321, essential for productive biofilm and influences virulence and pathogenesisThe transposon insertion mutation of regulator PA3321 (PA3321∷Tn) significantly reduced biofilm formation, while enhancing virulence in Drosophila melanogaster fruit fly and BALB/C mouse infection models. This regulator influenced bacterial motility, c-di-GMP signaling, and the RhlI-RhlR and Pseudomonas quinolone signal (PQS) quorum-sensing (QS) systems. We used RNA-seq assays to identify the regulatory mechanisms of PA3321. We observed that 391 genes were up-regulated and 114 genes were down-regulated in the absence of PA3321, many of which are involved in rhamnolipid synthesis, type II, type III, and type VI secretion systems, and QS systems.2026/06/16GSE335556GSM9815495GSM9815496GSM9815493GSM9815494GSM9815491GSM981549218287335556Pseudomonas aeruginosa