{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE335nnn/GSE335951/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Homo sapiens"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE335951"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"RamDA-seq analysis of CRISPRa-mediated RELA activation for proof-of-concept validation of CATaN","description":"This dataset was generated to validate CATaN (Canonical correlation Analysis of Transcriptome and TF-gene regulatory Networks), an unsupervised framework that integrates transcriptomic data with transcription factor (TF) gene regulatory networks. To test whether CATaN correctly recovers the transcriptional program driven by a specific TF, we activated RELA, a subunit of NF-κB, in Jurkat cells using CRISPR activation (CRISPRa; dCas9-VP64 with a single guide RNA targeting RELA) and compared it with a control guide RNA. Transcriptomes were profiled by RamDA-seq. Transductions were performed in triplicate for each condition, and cells were harvested at five, six, and seven days post-transduction. Differential expression analysis showed concordant directional changes in RELA-regulated genes; CATaN applied to these data assigned higher CC1 loadings to RELA ChIP-seq samples than to other TFs. Together, these results provide proof-of-concept support for the method.","dates":{"publication":"2026/06/29"},"accession":"GSE335951","cross_references":{"GSM":["GSM9824149","GSM9824167","GSM9824145","GSM9824168","GSM9824146","GSM9824169","GSM9824147","GSM9824148","GSM9824141","GSM9824163","GSM9824164","GSM9824142","GSM9824165","GSM9824143","GSM9824144","GSM9824166","GSM9824160","GSM9824161","GSM9824162","GSM9824140","GSM9824139","GSM9824156","GSM9824157","GSM9824158","GSM9824159","GSM9824152","GSM9824174","GSM9824153","GSM9824154","GSM9824155","GSM9824170","GSM9824171","GSM9824172","GSM9824150","GSM9824173","GSM9824151"],"GPL":["34284"],"GSE":["335951"],"taxon":["Homo sapiens"]}}