GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE335nnn/GSE335951/primary200OKTranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE335951GEOGSEfalseRamDA-seq analysis of CRISPRa-mediated RELA activation for proof-of-concept validation of CATaNThis dataset was generated to validate CATaN (Canonical correlation Analysis of Transcriptome and TF-gene regulatory Networks), an unsupervised framework that integrates transcriptomic data with transcription factor (TF) gene regulatory networks. To test whether CATaN correctly recovers the transcriptional program driven by a specific TF, we activated RELA, a subunit of NF-κB, in Jurkat cells using CRISPR activation (CRISPRa; dCas9-VP64 with a single guide RNA targeting RELA) and compared it with a control guide RNA. Transcriptomes were profiled by RamDA-seq. Transductions were performed in triplicate for each condition, and cells were harvested at five, six, and seven days post-transduction. Differential expression analysis showed concordant directional changes in RELA-regulated genes; CATaN applied to these data assigned higher CC1 loadings to RELA ChIP-seq samples than to other TFs. Together, these results provide proof-of-concept support for the method.2026/06/29GSE335951GSM9824149GSM9824167GSM9824145GSM9824168GSM9824146GSM9824169GSM9824147GSM9824148GSM9824141GSM9824163GSM9824164GSM9824142GSM9824165GSM9824143GSM9824144GSM9824166GSM9824160GSM9824161GSM9824162GSM9824140GSM9824139GSM9824156GSM9824157GSM9824158GSM9824159GSM9824152GSM9824174GSM9824153GSM9824154GSM9824155GSM9824170GSM9824171GSM9824172GSM9824150GSM9824173GSM982415134284335951Homo sapiens