{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE336nnn/GSE336031/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE336031"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Conditional overexpression of PPARα in intestinal epithelium diminishes GIP enteroendocrine cells and circulating hormone levels","description":"PPARα transcriptional programs have tissue-dependent features, yet there is a lack of genetic research tools to study tissue-specific signaling activation without the addition of a systemic agonist. Our aim was to investigate intestinal epithelial-specific roles of PPARα signaling using a novel transgenic mouse that enables spatial and temporal control of Ppara overexpression. CAG-Ppara,-EGFP mice were bred to Villin-CreERT2 to establish the IEC-Ppara mouse, which was compared to littermate controls 2 weeks after tamoxifen exposure. Transcriptional analysis of intestinal tissue from IEC-Ppara mice showed upregulation of PPARα target genes and functional enrichment for fatty acid catabolic processes. Unexpectedly, the enteroendocrine hormone Gip was among the most downregulated genes. GIP-positive enteroendocrine cell number, GIP circulating hormone, and the incretin response were also reduced in IEC-Ppara mice. These findings reveal PPARα as a regulator of GIP and support a new framework in which PPARα signaling influences glucose homeostasis via a gut hormone axis","dates":{"publication":"2026/08/14"},"accession":"GSE336031","cross_references":{"GSM":["GSM9825476","GSM9825487","GSM9825488","GSM9825477","GSM9825489","GSM9825478","GSM9825479","GSM9825483","GSM9825484","GSM9825473","GSM9825474","GSM9825485","GSM9825486","GSM9825475","GSM9825490","GSM9825480","GSM9825481","GSM9825482"],"GPL":["24247"],"GSE":["336031"],"taxon":["Mus musculus"],"PMID":["[42595008]"]}}