GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE336nnn/GSE336056/primaryOK200TranscriptomicsHomo sapiens OtherExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE336056GEOGSEfalseA single chromosome 3p break initiates clear cell renal cell carcinoma evolutionWe used single-cell RNA sequencing (10x Genomics) coupled with CITE-seq cell hashing (HTO) to profile and compare the transcriptomes of renal proximal tubule epithelial cells (RPTECs) after different CRISPR-Cas9 ribonucleoprotein (RNP) perturbations. Cells were treated with a non-targeting control sgRNA (sgNTC), an sgRNA targeting chromosome 3p (sg3p), or an sgRNA targeting chromosome Yq (sgYq), and harvested at Day 5 and Day 10. Hashtag-labeled samples were pooled into three 10x Chip G lanes, and gene-expression and HTO libraries were sequenced on an Illumina NovaSeq X Plus. Copy-number alterations were inferred (inferCNV) to identify cells with chromosome 3p loss, and differential gene expression was compared between 3p-loss and no-loss populations over time.2026/09/21GSE336056GSM9826218GSM9826217GSM9826214GSM9826213GSM9826216GSM982621534284336056Homo sapiens[42465403]