{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE336nnn/GSE336129/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Arabidopsis thaliana"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE336129"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"The 11-hydroxy-jasmonic acid pathway expands jasmonate catabolic repertoire for stress-specific hormone attenuation in Arabidopsis","description":"Jasmonates are plant hormones undergoing complex metabolic conversions, generating organ- or stimulus-specific signatures. Several jasmonates result from catabolism of the master regulator jasmonoyl-isoleucine (JA-Ile) that controls most jasmonate responses upon developmental or environmental cues. Hydroxylation of the precursor jasmonic acid by Jasmonic Acid Oxidases (JAO) holds a peculiar regulatory function, by attenuating basal JA-Ile formation and action, and promoting growth. Here we re-investigated the nature and origins of hydroxy-JAs and their derivatives in Arabidopsis. Using advanced analytical methods and pathway-impaired mutants, we show that JAO enzymes produce exclusively 11OH-JA and are preferentially recruited in response to fungal infection where 11-O-Glc-JA accumulates as its main glucosylated derivative. Conversly, mechanical wounding triggers the dominant formation of 12-OH-JA (and its derivatives 12-O-Glc-JA and 12-HSO4-JA) as a cleavage product of the JA-Ile catabolite 12OH-JA-Ile by amidohydrolase activity. Our findings elucidate the 11OH-JA biosynthetic pathway and reveal two distinct enzyme systems generating position-specific hydroxy-JA isomers in response to stress. Direct treatment of seedlings with 11OH-JA or 12OH-JA did not trigger specific transcriptional changes, indicating these compounds are inactive derivatives.","dates":{"publication":"2026/08/25"},"accession":"GSE336129","cross_references":{"GSM":["GSM9828219","GSM9828217","GSM9828228","GSM9828229","GSM9828218","GSM9828226","GSM9828215","GSM9828227","GSM9828216","GSM9828235","GSM9828224","GSM9828213","GSM9828214","GSM9828225","GSM9828236","GSM9828222","GSM9828233","GSM9828234","GSM9828223","GSM9828231","GSM9828220","GSM9828232","GSM9828221","GSM9828230"],"GPL":["34544"],"GSE":["336129"],"taxon":["Arabidopsis thaliana"]}}