GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE336nnn/GSE336305/primary200OKOtherMus musculusOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE336305GEOGSEfalseGenome-wide CRISPR screening on cytotoxic T lymphocytes mediated cell killing independent of Casp3, Mlkl, and BidTo gain insight into cytotoxic lymphocyte–mediated, Casp3, necroptosis, and Mitochondrial outer membrane permeabilization (MOMP) independent cancer cell death, we leveraged the a CTL–αCD3–P815 killing system and performed a genome-wide CRISPR screen. Cas9 expressing and Casp3-/-Mlkl-/-Bid-/- mouse mastocytoma P815 cells were first transduced with Brie gRNA library and subjected to cytotoxic T lymphocytes-mediated selection. Surviving cells were collected and sequenced to identify genes/gRNAs enriched following selection.2026/06/26GSE336305GSM9831793GSM9831792GSM9831795GSM9831794GSM9831797GSM983179634328336305Mus musculus