GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE336nnn/GSE336428/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE336428GEOGSEfalsedCas9-KRAB CRISPRi-mediated transcriptional repression of TERT in H1299 non-small cell lung cancer cellsBulk RNA sequencing was performed to assess transcriptome-wide changes associated with dCas9-KRAB mediated CRISPR interference (CRISPRi) of telomerase reverse transcriptase (TERT) in H1299 non-small cell lung cancer cells. H1299 cells were transduced with a lentiviral vector co-expressing dCas9-KRAB and either no guide RNA (non-targeting control) or a TERT-promoter-targeting single-guide RNA, selected with puromycin, and harvested at day 33 after transduction. Differential expression analysis was used to evaluate the specificity of sustained TERT repression.2026/06/24GSE336428GSM9835013GSM9835011GSM9835012GSM983501024676336428Homo sapiens