GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE336nnn/GSE336449/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE336449GEOGSEfalseIntrasplenic Thymus Organogenesis From Injectable Tissue Fragments Restores Functional T-cell immunity [RNA-seq_2]Thymic dysfunction due to congenital defects, aging, infection, or cytotoxic therapies causes profound restriction of the T-cell receptor (TCR) repertoire and impaired adaptive immunity. Restoring thymic function is therefore critical for affected patients. Here, we describe intrasplenic thymus regeneration (ISTR), an approach in which cultured thymic tissue fragments are implanted into the spleens of athymic nude and humanized mice. The spleen’s abundant vasculature and supportive stromal milieu enable rapid thymic organogenesis, leading to reconstitution of peripheral T-cell compartments with normal phenotypes and functional competence. Relative to intramuscular implantation, ISTR drives faster and more complete thymic development, generates greater T-cell output, and is associated with a lower incidence of graft-versus-host disease. Functional readouts show restored T-cell immunity, including protective responses to viral challenge and tumor models. In humanized animals, splenic thymus grafts also promote rapid emergence of functional human T cells. These results identify the spleen as an optimal ectopic niche for thymus regeneration and provide a promising strategy for clinical immune reconstitution and regenerative immunology.2026/09/02GSE336449GSM9835610GSM9835614GSM9835613GSM9835612GSM9835611GSM9835616GSM9835615GSM9835609GSM983560824247336449Mus musculus[42640144]