GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE336nnn/GSE336575/primaryOK200TranscriptomicsRattus norvegicusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE336575GEOGSEfalseA Rat Embryonic Enteric Nervous System Culture Platform for Modeling Neuronal Subtype Diversity and Network Activity MaturationWe present an in vitro culture system of enteric neurons and glia derived from migratory cells isolated from the embryonic rat small intestine. This system gives rise to functional neuronal networks composed of multiple enteric neuronal subtypes and closely integrated with enteric glial cells. Single-cell RNA sequencing identifies diverse neuronal populations, including ChAT-positive excitatory neurons, NOS-positive inhibitory neurons, and VIP-positive interneurons, as well as a glial cell population. Transcriptomic analysis further defines cell type-specific marker profiles and candidate regulators of enteric neuronal and glial development. Electrophysiological recordings demonstrate maturation of spontaneous network activity, with contributions from cholinergic, nitrergic, and VIPergic neuronal populations. Together, these findings establish a tractable in vitro platform for investigating neuronal subtype specification, the emergence of cellular diversity, neuro-glial interactions and functional electrophysiological network organization.2026/06/30GSE336575GSM983760034877336575Rattus norvegicus