GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE336nnn/GSE336602/primary200OKTranscriptomicsClostridioides difficileExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE336602GEOGSEfalseEffect of curing the phi027 prophage for gene expresson in Clostridioides difficile clinical isolate 500/12Clostridioides difficile is a clinically important nosocomial pathogen whose virulence is primarily associated with toxin production and can be influenced by prophage carriage. In this study, we investigated the molecular basis of phi027-dependent phenotypic differences previously observed between the clinical RT176 strain 500/12 and its prophage-free derivative CKH08. Comparative transcriptomic analysis revealed that loss of phi027 was associated with reduced expression of the sinR/R' operon, downregulation of multiple flagellar biosynthesis genes.2026/07/01GSE336602GSM9838636GSM9838637GSM9838638GSM9838639GSM9838643GSM9838640GSM9838641GSM983864230071336602Clostridioides difficile