GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE336nnn/GSE336612/primary200OKTranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE336612GEOGSEfalseEffects of bile acid exposure on gene expression in oesophageal epithelial cellsGastro-oesophageal reflux disease is highly prevalent in systemic sclerosis (SSc) and may contribute to oesophageal tissue damage. To investigate the molecular effects of reflux-associated injury, human oesophageal epithelial cells (Het1A) were exposed to bile acids and analysed by RNA sequencing. Chronic bile acid exposure induced transcriptional changes associated with innate immune activation, type I interferon signalling, TGF-β responses, epithelial–mesenchymal transition (EMT), oxidative stress, and mitochondrial dysfunction. Differential gene expression profiles were compared with publicly available SSc oesophageal transcriptomic datasets to identify shared disease-associated pathways. These data provide insight into the molecular mechanisms by which bile acid-induced epithelial injury may contribute to oesophageal pathology in systemic sclerosis.2026/06/30GSE336612GSM9838820GSM9838821GSM9838816GSM9838817GSM9838818GSM98388199052336612Homo sapiens