GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE336nnn/GSE336658/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE336658GEOGSEfalseFibroblast-driven transcriptional reprogramming in response to doxorubicin in heterotypic melanoma spheroidsBulk RNA-sequencing was used to compare the transcriptional response to doxorubicin (DOX) between two multicellular melanoma spheroid models: a baseline model composed of B16.F10 melanoma cells, 2H11 endothelial cells, and bone marrow-derived macrophages (BEM), and a fibroblast-containing counterpart (BEMF) additionally incorporating primary murine dermal fibroblasts. Spheroids of each model were exposed for 48 h to their respective IC30 concentration of doxorubicin (0.56 microM for BEM; 0.79 microM for BEMF) or left untreated as controls, in two independent biological replicates per model/treatment combination. RNA-seq was performed to identify differentially expressed genes associated with fibroblast-dependent modulation of the doxorubicin response in melanoma spheroids.2026/06/30GSE336658GSM9840071GSM9840072GSM9840070GSM9840068GSM9840069GSM9840066GSM9840067GSM984007324247336658Mus musculus