{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE336nnn/GSE336743/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Other"],"species":["feces metagenome"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE336743"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Do intestinal parasites alter the fecal microbiome in dogs?","description":"Pets serve as companion animals and provide emotional support for people worldwide. These animals are also known to harbor diverse zoonotic pathogens, including parasites. In the dog gut, bacteria can coexist with intestinal helminths (e.g., Trichuris vulpis, Ancylostoma caninum, Toxocara canis), establishing interactions that may be particularly relevant in regions where helminth infections are endemic. Thus, their close contact with humans makes them important in the transmission of pathogens responsible for significant diseases. Most bacterial sequences identified in the canine gastrointestinal (GI) tract fall into five phyla: Bacillota, Fusobacteriota, Bacteriota, Pseudomonadota, and Actinobacteriota. In the present study, the main objective was to investigate the association of T. vulpis in dogs with changes in their microbiota, thereby comparing the microbiota of dogs infected with this and/or other species of parasitic nematodes with dogs with non-helminth parasites. The microbiota analysis was performed on fecal samples from dogs parasitized by T. vulpis and/or other intestinal helminths. The initial individual analysis revealed high variability among dogs with Phylum Bacillota being the most abundant in most samples. Initial analyses revealed a characteristic profile in dogs positive for T. vulpis: a dominant abundance of Bacillota, followed at much lower levels by Pseudomonadota and Actinomycetota, while Fusobacteriota and Bacteroidota were nearly absent. Within Bacillota, Clostridia was the predominant class, particularly the family Lachnospiraceae. The most abundant genera included Ruminococcus, Cupriavidus, Collinsella, and Peptosclostridium. In contrast, dogs parasitized only with hookworms exhibited a higher proportion of Fusobacteriota and Bacillota, and lower Bacteroidota. The class Fusobacteriia was most abundant, followed by Clostridia, with Fusobacteriaceae as the dominant family and Fusobacterium, Bacteroides, and Blautia as the predominant genera. The negative control group (NC) exhibited all five phyla, with Bacillota being the most abundant. The classes Clostridia and Fusobacteriia were the most prominent. Dogs infected exclusively with T. vulpis exhibited a distinct fecal microbiota characterized by Bacillota dominance, marked reductions in Fusobacteriota and Bacteroidota, and lower alpha diversity compared with non-infected dogs. In contrast, hookworm infections were associated with relatively minor alterations in fecal microbial composition. These findings could suggest that T. vulpis infection is associated with intestinal dysbiosis and a more pronounced restructuring of the canine gut microbiota than other intestinal nematodes examined in this study. This suggestion would be enforced by the reduced abundance of health-associated genera such as Blautia and Fusobacterium observed in dogs with trichuriasis. No substantial changes were detected in the fecal microbiota of dogs parasitized with both hookworms and non-infected dogs, likely due to the location of these parasites in the small intestine, as changes in the microbiota of the large intestine are those detected in the highest percentage in feces.","dates":{"publication":"2026/09/25"},"accession":"GSE336743","cross_references":{"GSM":["GSM9841606","GSM9841605","GSM9841604","GSM9841602","GSM9841589","GSM9841600","GSM9841587","GSM9841585","GSM9841583","GSM9841582","GSM9841580","GSM9841609","GSM9841608","GSM9841607","GSM9841616","GSM9841615","GSM9841614","GSM9841613","GSM9841612","GSM9841578","GSM9841611","GSM9841610","GSM9841599","GSM9841576","GSM9841597","GSM9841574","GSM9841595","GSM9841573","GSM9841572","GSM9841593","GSM9841571","GSM9841570","GSM9841591"],"GPL":["32080"],"GSE":["336743"],"taxon":["feces metagenome"]}}