GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE336nnn/GSE336865/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE336865GEOGSEfalseNootkatone preferentially suppressed the AKT/mTOR signaling in activated fibroblastsHaving established that nootkatone restores autophagic flux and that autophagy contributes functionally to its anti-fibrotic activity, we next sought to identify the upstream events associated with this response. Transcriptomic profiling was therefore performed in TGF-β1–activated lung fibroblasts treated with or without nootkatone. RNA-seq analysis revealed clear separation among control, TGF-β1-treated, and nootkatone + TGF-β1-treated fibroblasts by principal component analysis, indicating distinct transcriptional states across groups。Pathway enrichment analysis of genes downregulated by nootkatone revealed significant enrichment of PI3K-Akt signaling and focal adhesion–PI3K-Akt-mTOR signaling, together with matrix-adhesion and cytoskeletal programs, including ECM-receptor interaction, focal adhesion, and cytoskeleton in muscle cells2026/08/17GSE336865GSM9843892GSM9843893GSM9843894GSM9843895GSM9843896GSM9843897GSM9843898GSM9843899GSM984390034284336865Homo sapiens