GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE336nnn/GSE336961/primaryOK200TranscriptomicsHomo sapiensExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE336961GEOGSEfalse冷冻化处理和通道依赖性恢复下HeLa细胞的RNA序列分析This study performed RNA sequencing to investigate transcriptional changes in HeLa cells under cryosilicification treatment (Si20 and Si30) and in untreated controls. Cells were profiled at early response stage (P0, 0 h and 6 h) and during passage-dependent recovery (P1–P3). Differential gene expression and pathway analyses were conducted to characterize cellular responses to cryosilicification and to evaluate the persistence of transcriptional changes across cell passages. All samples were sequenced using Illumina NovaSeq platforms (NovaSeq 6000 and NovaSeq X Plus), and both raw sequencing data and processed expression matrices are provided.2026/06/29GSE336961GSM9845420GSM9845422GSM9845400GSM9845421GSM9845424GSM9845402GSM9845401GSM9845423GSM9845404GSM9845403GSM9845406GSM9845405GSM9845408GSM9845407GSM9845409GSM9845411GSM9845410GSM9845399GSM9845413GSM9845412GSM9845415GSM9845414GSM9845417GSM9845416GSM9845419GSM98454183428424676336961Homo sapiens