{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE336nnn/GSE336999/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE336999"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Whole-transcriptome sequencing of NT2.5, NT2.5LM, and NT2.5LV: a HER2-positive murine breast cancer cell line series with lung- and liver-preferential metastasis","description":"The NT2.5 cell line and its lung-preferential (NT2.5LM) and liver-preferential (NT2.5LV) metastatic derivatives constitute an isogenic HER2-positive murine breast cancer model derived from neu-N transgenic FVB/N mice. To define the transcriptional programs associated with organ-preferential metastatic colonization, we performed bulk poly(A) RNA-sequencing on the three cell lines grown in vitro. Differential expression analysis identified divergent, organ-correlated transcriptional programs distinguishing the lung- and liver-preferential derivatives on a conserved genomic background. These data accompany the whole-genome sequencing of the same cell line series deposited under the same BioProject.","dates":{"publication":"2026/08/08"},"accession":"GSE336999","cross_references":{"GSM":["GSM9846533","GSM9846532","GSM9846535","GSM9846534","GSM9846537","GSM9846536","GSM9846539","GSM9846538"],"GPL":["34290"],"GSE":["336999"],"taxon":["Mus musculus"]}}