GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE336nnn/GSE336999/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE336999GEOGSEfalseWhole-transcriptome sequencing of NT2.5, NT2.5LM, and NT2.5LV: a HER2-positive murine breast cancer cell line series with lung- and liver-preferential metastasisThe NT2.5 cell line and its lung-preferential (NT2.5LM) and liver-preferential (NT2.5LV) metastatic derivatives constitute an isogenic HER2-positive murine breast cancer model derived from neu-N transgenic FVB/N mice. To define the transcriptional programs associated with organ-preferential metastatic colonization, we performed bulk poly(A) RNA-sequencing on the three cell lines grown in vitro. Differential expression analysis identified divergent, organ-correlated transcriptional programs distinguishing the lung- and liver-preferential derivatives on a conserved genomic background. These data accompany the whole-genome sequencing of the same cell line series deposited under the same BioProject.2026/08/08GSE336999GSM9846533GSM9846532GSM9846535GSM9846534GSM9846537GSM9846536GSM9846539GSM984653834290336999Mus musculus