{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE337nnn/GSE337112/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Other"],"species":["Mus musculus"],"gds_type":["Other"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE337112"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"MeRIP-seq profiling of m⁶A modifications in Alkbh5-KO and control bone marrow-derived macrophages following M1 polarization","description":"We performed MeRIP-seq combined with RNA-seq to identify downstream targets of the m⁶A demethylase ALKBH5 in macrophages. Bone marrow-derived macrophages from myeloid-specific Alkbh5 knockout and Alkbh5-fl/fl littermate control mice were polarized to the M1 phenotype with LPS and IFN-γ. m⁶A-enriched RNA was immunoprecipitated using an anti-m⁶A antibody and sequenced on an Illumina NovaSeq 6000 platform. Altered m⁶A peaks in Alkbh5-KO cells were predominantly located within coding sequences, 3′ untranslated regions, and regions near stop codons. Integrative analysis with RNA-seq identified genes with both altered m⁶A methylation and differential expression, revealing the m⁶A epitranscriptomic landscape regulated by ALKBH5 in M1-polarized macrophages.","dates":{"publication":"2026/09/03"},"accession":"GSE337112","cross_references":{"GSM":["GSM9848347","GSM9848336","GSM9848337","GSM9848345","GSM9848346","GSM9848338","GSM9848339","GSM9848340","GSM9848343","GSM9848344","GSM9848341","GSM9848342"],"GPL":["24247"],"GSE":["337112"],"taxon":["Mus musculus"]}}