GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE337nnn/GSE337112/primaryOK200OtherMus musculusOtherhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE337112GEOGSEfalseMeRIP-seq profiling of m⁶A modifications in Alkbh5-KO and control bone marrow-derived macrophages following M1 polarizationWe performed MeRIP-seq combined with RNA-seq to identify downstream targets of the m⁶A demethylase ALKBH5 in macrophages. Bone marrow-derived macrophages from myeloid-specific Alkbh5 knockout and Alkbh5-fl/fl littermate control mice were polarized to the M1 phenotype with LPS and IFN-γ. m⁶A-enriched RNA was immunoprecipitated using an anti-m⁶A antibody and sequenced on an Illumina NovaSeq 6000 platform. Altered m⁶A peaks in Alkbh5-KO cells were predominantly located within coding sequences, 3′ untranslated regions, and regions near stop codons. Integrative analysis with RNA-seq identified genes with both altered m⁶A methylation and differential expression, revealing the m⁶A epitranscriptomic landscape regulated by ALKBH5 in M1-polarized macrophages.2026/09/03GSE337112GSM9848347GSM9848336GSM9848337GSM9848345GSM9848346GSM9848338GSM9848339GSM9848340GSM9848343GSM9848344GSM9848341GSM984834224247337112Mus musculus