{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE337nnn/GSE337368/"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE337368"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"Investigating the effects of IFN-gamma on attached and detached ovarian cancer cells","description":"The interplay between intrinsic and niche-driven mechanisms that enables DTCs to survive and home to distant organs remains incompletely understood. Single-cell RNA sequencing (scRNA-seq) coupled with velocity analyses in ascites and metastasis-bearing omenta uncovered an emergent and distinct interferon-gamma centric transcriptional trajectory, enriched among early seeding clones. Knockout of IFN-gamma receptor 1 (Ifngr1) in tumor cells significantly reduced metastatic burden and extended survival, underscoring the importance of tumor cell intrinsic IFN-gamma signaling in ovarian cancer metastasis. We identified that the tumor intrinsic IFN-gamma response and ascites-derived tumor-associated macrophages (TAMs) protect cancer cells from anoikis-mediated death. To investigate the mechanism underlying increased cancer cell survival under IFN-gamma treatment and ULA conditions, we performed bulk RNA-sequencing. We discovered increased expression of the anti-apoptotic gene Parp14 in cancer cells treated with IFN-gamma, specifically under ultra low attachment conditions.","dates":{"publication":"2026/07/04"},"accession":"GSE337368","cross_references":{"GSM":["GSM9853859","GSM9853879","GSM9853868","GSM9853858","GSM9853869","GSM9853877","GSM9853866","GSM9853867","GSM9853878","GSM9853864","GSM9853875","GSM9853876","GSM9853865","GSM9853862","GSM9853873","GSM9853874","GSM9853863","GSM9853871","GSM9853860","GSM9853861","GSM9853872","GSM9853880","GSM9853881","GSM9853870"],"GPL":["34290"],"GSE":["337368"],"taxon":["Mus musculus"]}}