GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE337nnn/GSE337368/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE337368GEOGSEfalseInvestigating the effects of IFN-gamma on attached and detached ovarian cancer cellsThe interplay between intrinsic and niche-driven mechanisms that enables DTCs to survive and home to distant organs remains incompletely understood. Single-cell RNA sequencing (scRNA-seq) coupled with velocity analyses in ascites and metastasis-bearing omenta uncovered an emergent and distinct interferon-gamma centric transcriptional trajectory, enriched among early seeding clones. Knockout of IFN-gamma receptor 1 (Ifngr1) in tumor cells significantly reduced metastatic burden and extended survival, underscoring the importance of tumor cell intrinsic IFN-gamma signaling in ovarian cancer metastasis. We identified that the tumor intrinsic IFN-gamma response and ascites-derived tumor-associated macrophages (TAMs) protect cancer cells from anoikis-mediated death. To investigate the mechanism underlying increased cancer cell survival under IFN-gamma treatment and ULA conditions, we performed bulk RNA-sequencing. We discovered increased expression of the anti-apoptotic gene Parp14 in cancer cells treated with IFN-gamma, specifically under ultra low attachment conditions.2026/07/04GSE337368GSM9853859GSM9853879GSM9853868GSM9853858GSM9853869GSM9853877GSM9853866GSM9853867GSM9853878GSM9853864GSM9853875GSM9853876GSM9853865GSM9853862GSM9853873GSM9853874GSM9853863GSM9853871GSM9853860GSM9853861GSM9853872GSM9853880GSM9853881GSM985387034290337368Mus musculus