{"database":"GEO","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Other":["ftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE337nnn/GSE337703/"]},"type":"primary"},"statusCodeValue":200,"statusCode":"OK"}],"scores":null,"additional":{"omics_type":["Transcriptomics"],"species":["Mus musculus"],"gds_type":["Expression profiling by high throughput sequencing"],"full_dataset_link":["https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE337703"],"repository":["GEO"],"entry_type":["GSE"],"additional_accession":[]},"is_claimable":false,"name":"p300/CBP inhibition promotes apoptosis in mouse embryos by impairing H1K75ac-mediated DNA repair from the 4-cell to blastocyst stage","description":"Defective pre-implantation embryonic development is a major cause of implantation failure and early embryonic arrest. p300/CBP is known to influence blastocyst formation; nevertheless, its molecular regulatory cascades mediating murine embryonic development from the 4-cell to blastocyst stage are poorly understood. Here, we show that pharmacological inhibition of p300/CBP markedly reduced morula and blastocyst rate, increased embryonic fragmentation, and disrupted markers of compaction, polarization, and first lineage segregation from the 4-cell to blastocyst stage. Smart-seq2 analysis showed that differentially expressed genes were enriched in pathways related to apoptosis, cell-cycle regulation, DNA modification, and embryonic development. Consistently, immunofluorescence analyses showed that p300/CBP inhibition increased apoptosis, suppressed cell proliferation, and promoted DNA damage accumulation. Mechanistically, p300/CBP inhibition reduced histone H1 lysine 75 acetylation (H1K75ac), leading to impaired DNA repair and subsequent activation of p38 signaling, thereby triggering apoptosis. Collectively, our findings identify a p300/CBP-H1K75ac-DNA repair-p38 MAPK signaling axis that maintains genome integrity and developmental competence in mouse pre-implantation embryos.","dates":{"publication":"2026/07/08"},"accession":"GSE337703","cross_references":{"GSM":["GSM9859733","GSM9859732","GSM9859735","GSM9859734","GSM9859737","GSM9859736","GSM9859739","GSM9859738"],"GPL":["30215"],"GSE":["337703"],"taxon":["Mus musculus"]}}