GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE337nnn/GSE337822/primaryOK200TranscriptomicsMus musculusExpression profiling by high throughput sequencinghttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE337822GEOGSEfalseTranscriptomic Signatures Reveal Overlapping Shared and DistinctYet State-Specific Preoptic Neuronal Ensembles Activated by Anesthesia and SleepGeneral anesthesia and non-rapid-eye-movement (NREM) sleep share overlapping neuronal circuitry within the preoptic area (POA), yet they are fundamentally distinct states. We performed single nucleus RNA sequencing (snRNA-seq) on the POA from adult C57BL/6J mice after 30-minutes of breathing either 1.2% isoflurane in oxygen or an oxygen control. Cell clusters were identified by projecting to the existing cellular taxonomy established in our previously published POA snRNA-seq dataset of recovery sleep (GEO: GSE243489) and transcriptional changes in rapid primary response genes (rPRGs) was used to define isoflurane-activated or isoflurane-inhibited clusters. Finally, multiplex fluorescence in situ hybridization was performed to independently corroborate the snRNA-seq results.2026/07/07GSE337822GSM9861028GSM9861017GSM9861027GSM9861016GSM9861015GSM9861026GSM9861025GSM9861019GSM9861018GSM9861029GSM9861031GSM9861020GSM9861030GSM9861024GSM9861023GSM9861034GSM9861033GSM9861022GSM9861021GSM986103224247337822Mus musculus