GEOapplication/xmlftp://ftp.ncbi.nlm.nih.gov/geo/series/GSE337nnn/GSE337899/primaryOK200Methylation profilingHomo sapiensMethylation profiling by genome tiling arrayhttps://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE337899GEOGSEfalseMethylomic Analysis of Nasal Brushings Poorly Discriminates Subgroups in Pediatric Acute Respiratory Distress SyndromePediatric acute respiratory distress syndrome (PARDS) is biologically heterogeneous. To determine whether the nasal epithelial methylome identifies subgroups of PARDS, we profiled genome-wide DNA methylation in nasal brushings from 21 children with PARDS and 3 pediatric ICU controls using the Infinium MethylationEPIC v2.0 array. After estimating and adjusting for cell-type composition, we performed unsupervised clustering and cell-type-specific differential methylation analysis. Cellular composition explained the majority of variation in the unadjusted methylome, and composition-adjusted clustering yielded only weak subgroup structure. These data indicate that, at this sample size, the nasal methylome poorly discriminates PARDS subgroups.2026/07/07GSE337899GSM9862490GSM9862502GSM9862501GSM9862500GSM9862489GSM9862499GSM9862488GSM9862503GSM9862494GSM9862483GSM9862482GSM9862493GSM9862492GSM9862481GSM9862491GSM9862480GSM9862487GSM9862498GSM9862497GSM9862486GSM9862496GSM9862485GSM9862484GSM986249533022337899Homo sapiens